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What do you know about some underlying principles of chromatography discuss one of them in detail?

What do you know about some underlying principles of chromatography discuss one of them in detail?
What do you know about some underlying principles of chromatography discuss one of them in detail?
Different basic principles are given as follows
Distribution value
Resolution value
Peak shape
Efficiency

Here Distribution value is discussed in detail as follows:
Distribution value Distribution value can be defined as follows
“Relationship which explains the relationship between concentration of solute maintained by one phase and attached to another phase”.
General representation of distribution factor
Distribution value can be represented as follows
Symbolic representation
K=distribution factor
K=C1/C2
C1=concetration of the solute in phase 1
C2=Concentration of the solute in phase 2
Modification of distribution equation
This equation can be limited to a someone special type of chromatography technique.
Distribution factor is dependent upon
Temperature
Pressure
Distribution value is independent upon the concentration.
However in practice distribution value is independent upon amount of concentration in certain cases.It Is seen over a limited range of chromatography technique.
Discuss the Distribution value in case of partition method?
In partition method distribution co-efficient is called partition method.This also applies in situation with solvent extraction.
In this case
C1 is changed into concentration of stationary phase
C2 is changed into concentration of mobile phase
They are changed into Cs Cm
Cs=Concentration of the stationary phase
Cm=Concentration of the mobile phase
Kp=Cs/Cm
Kp= Distribution co efficient in case of partition co -efficient

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